Superoxide anion radical scavenging activities of herbs. Therefore, in presence of superoxide desmutase that uses superoxide anion radicals as substrate, one would expect decrease in the formation of nitrite, formazan or reduction of cytochrome c. Free radical scavengers definition of free radical. Scavenging activities of these plants are comparable with standards. Superoxide radical scavenging activity by alkaline dmso method superoxide radical scavenging activity of the extracts were determined by alkaline dmso method according to the method of elizabeth and rao 1990 with slight modification in this method. The objective of this study was to compare the free radical scavenging activity of various compounds to their ability to inhibit lipid oxidation in foods. Superoxide is produced as a byproduct of oxygen metabolism and, if not regulated, causes many types of cell damage. Hydrogen peroxide is also damaging and is degraded by other. A part of the oxygen taken into living cells is changed to several harmful reactive oxygen species and free radicals. Evaluation of abelmoschus moschatus extracts for antioxidant. Evaluation of free radical scavenging activity of various leaf extracts from. Superoxide anion radical activity was measured by the.
Superoxide scavenging activity 10 superoxide anions were generated in samples that contained in 3. Antioxidant, free radical scavenging and antibacterial. As positive controls, epicatechin and lascorbic acid were also examined for dpph radical scavenging activity. The original pyrogallol 1,2,3trihydroxybenzene method, which was developed specifically for superoxide dismutase, is now widely used for measuring superoxide scavenging of other antioxidants. It was suggested that their higher activity on superoxide anion and dpph free radical scavenging activity, reducing capacity and inhibition of lipid peroxidation seem to be related with the release of specific antioxidants such as low molecular weight protein fragment, peptide and free amino acid derived form chicken leg bone protein during heating treatment rather than the increase of peptide. The different extracts showed significant inhibition of superoxide radicals in a dosedependant manner. The antioxidant activity of the memq was evaluated by the. Superoxide anion and hydroxyl radical scavenging activities of. In vitro antioxidant activities of the plant extract were determined by dpph and no scavenging method. In vitro antioxidant and free radical scavenging activity.
Numerous attempts have been made to relate the free radical scavenging capacity of compounds to their antioxidant activity in foods even though antioxidant activity is dependent on both physical and chemical properties. Beyza ozturk sarikaya and ilhami gulcin affiliation. Antioxidant and free radical scavenging potential of citrullus colocynthis l. Abstractthe present study was designed to explore the flavonoid contents and. Based on dpph and hydroxyl radical scavenging activity, tpl showed strong scavenging activity 91. Radical scavenging and antioxidant activity of tannic acid. Chrysin, rutin and quercetin were run to explore the effect of. The extract was examined for different reactive oxygen species ros scavenging activities including hydroxyl, superoxide, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and. Radical scavenging and antioxidant capacity of serotonin volume. In vitro antioxidant and free radical scavenging activity 39 roots are the main portions of the whole plant as they possess wide number of the therapeutic agents. The antioxidant activity of the aqueous ethanolic extract of doum leaves, hyphaene thebaica l. The antioxidant activity of the tannic acid, bha, bht. Three extracts of seed amsi, amsii and amsiii and one leaf extract amli were found to be an efficient scavenger of superoxide radical generation.
Preliminary phytochemical screening of the plant showed the presence of large amounts of. Free radical scavenging activity of crude extracts and 4 bioline. In vitro evaluation of free radical scavenging activity of. Determination of free radical scavenging activity of plant. Daily subconjunctival injections were given in rabbit eyes after alkali burns. This study was designed to explore the antioxidant potential of hexane, chloroform, ethyl acetate, methanol, and water extract of bark and leaves of cassia siamea and cassia javanica by superoxide anion radical scavenging assay. Free radical scavenging activity, total phenolic content. Superoxide definition of superoxide by medical dictionary. Total antioxidant activity, metal chelation, radical scavenging dpph effects and reducing power as well as activities destructive to active oxygen species such as the superoxide anion radical, hydroxyl radical, and hydrogen peroxide are widely used for this purpose shimada et al. Dpph radical scavenging assay dpph radical scavenging activity was measured as per standard method using stable dpph radical7. The objective of the present study was to evaluate the antioxidant potential and free radical scavenging activity of a 70% methanol extract of s. Oxygen radical scavenging activity of flavonoidschemistry, metabolism, cardioprotective effects, and dietary sources. Abts was dissolved in deionized water to 7 mm concentration, and potassium. Reactive oxygen species ros, including free radicals such as superoxide anion radicalso.
Molecular oxygen dioxygen is a diradical containing two unpaired electrons, and. Measurement of superoxide anion scavenging activity was performed based on the method described by winterbourne et al 186. Superoxide anion radical scavenging activity superoxide radical o2 was generated from the photoreduction of riboflavin and was deducted by nitro blue tetrazolium dye nbt reduction method. Pdf hydrogen peroxide radical scavenging and total. The superoxide anion scavenging activity or sod like activity of mum212 strain was examined using an indirect colorimetric method 19160 sod assay kitwst, sigma aldrich that measures the formation of water soluble formazan dye upon the reduction of 24iodophenyl34nitrophenyl52,4disulfophenyl2htetrazolium, monosodium salt wst1. Among them, the 2,2diphenyl1picrylhydrazyl dpph spectrophotometric method is one of the most widely applied and is appreciated for its reliability. In addition, aafb melanin exhibited stronger scavenging activities on 2,2diphenyllpicrylhydrazyl dpph radical, superoxide radical and hydroxyl radical with. Invitro antioxidant and free radical scavenging activity. However, there has been no report on the antioxidant properties of this species. Cucurbitaceae is a medicinal plant traditionally used as an abortifacient and to treat constipation, oedema, bacterial infections, cancer and diabetes. By using hydrodynamic voltammetry, which generates the superoxide radical in situ, we show an unusual scavenging capability by embelin. Antioxidant and free radical scavenging potential of.
The percentages of radical scavenging property of the ethanolic, aqueous extract and parthenolide in this assay were 63. The method dpph is widely used for measurement of free radical scavenging ability of antioxidants perezjimenez and sauracalixto, 2008. In vitro antioxidant activities, free radical scavenging. For determination of radical scavenging activity of different foods, beverages and substrates were elaborated a. Nov, 2009 this study was designed to explore the antioxidant potential of hexane, chloroform, ethyl acetate, methanol, and water extract of bark and leaves of cassia siamea and cassia javanica by superoxide anion radical scavenging assay. Scavenging activity on dpph radical quantitative fig. Superoxide anion radical scavenging activity biology essay. Superoxide radical scavenging agents in treatment of. The results suggested that dwgps produced by fermentation could be used as a promising antioxidant ingredient. The original pyrogallol 1,2,3trihydroxybenzene method, which was developed specifically for superoxide dismutase, is now widely used for measuring superoxidescavenging of other antioxidants. The radical scavenging activity, antioxidant activity by carotene bleaching assay and, superoxide dismutase sod like ability decreased in the order of 70% methanol, 70% ethanol, cm, ea and, n.
There are numerous antioxidant methods for evaluation of antioxidant activity. Heat treatments application affected the phenolic contents of the seeds significantly p radical scavenging activity of the phenolics were done using 2,2diphenyl 1picrylhydrazyl dpph. An attempt was made to investigate the antioxidant activity of the methanolic extract of diplazium esculentum. Radical scavenging effects of different veronica species. Dpph free radical scavenging activity of the extracts of. Superoxide scavenging activity of the plant extract was determined by mccord and fridovich method, 1969 6 which depends on light induced superoxide generation by riboflavin and the corresponding reduction of ni troblue tetrazolium was measured spectrophotometrically at 560 nm. Antioxidant and free radical scavenging activities of. The present study evaluated the in vitro antioxidant and antibacterial activities of various extracts of aerial parts of periploca aphylla and ricinus communis. A comparative study on the antioxidant activity of. To test the hypothesis that superoxide radicals are involved in tissue destruction after alkali burns, superoxide dismutase, ascorbic acid, and glutathione were used as superoxide radical scavenging agents. Free radical scavenging activity of methanolic extract of. Antioxidant, antimicrobial, and free radical scavenging.
In this study, the influencing factors have been systematically investigated for the first time, and a number of experiments have proved that the ph is. Assay of nitric oxide scavenging activity nitric oxide scavenging activity can be estimated by the use of griess iliosvoy reaction garrat, 1964. Frsa free radical scavenging activity acronymfinder. The reaction mixture contained 50 mm phosphate buffer ph 7. Hydroxyl and superoxide anion radical scavenging activities of. Data obtained showed that the extract scavenged superoxide anion radicals ic 501602 gml in a dose dependant manner using. Embelin as a scavenger of superoxide is stronger than the common food additive antioxidant 2,6bis1,1dimethylethyl420 methylphenol, butylated hydroxytoluene, bht. Aug 17, 2011 superoxide radical scavenging activity. Free radical scavenging activity of seed of holarrhena.
To compare the antioxdant activity we used abts 2,2azinobis 3ethylbenzothiazoline6sulfonic acid diammonium radical scavenging assay which is applicable for both lipophilic and hydrophilic antioxidants. Reactive oxygen species ros, including the free radicals superoxide anion o 2. Assay for superoxide radical scavenging activity was based on the capacity of the sample to inhibit blue formazan formation by scavenging the superoxide radicals generated in riboflavinlightnbt system 5,10,12. An aqueous extract of this plant inhibits the citrus canker of lime 8. In vitro antioxidant and free radical scavenging activity of. Scavenging of hydroxyl radical was determined using dmpo. Chemical composition and radical scavenging activity of. Blumea balsamifera, flavonoids, leaf extracts, superoxide radical. The superoxide anion scavenging activity or sod like activity of mum212 strain was examined using an indirect colorimetric method 19160 sod assay kitwst, sigma aldrich that measures the formation of water soluble formazan dye upon the reduction of 24iodophenyl34nitrophenyl52,4disulfophenyl2htetrazolium, monosodium salt wst1 by. Free radical scavenging activity of phellinus merrillii 409 abts free radical scavenging assay total antioxidant status of the pm was measured using 2, 2. Superoxide radical o2 was generated from the photoreduction of riboflavin and was deducted by nitro blue tetrazolium dye nbt reduction method. Superoxide radical scavenging properties of extracts and.
Freeradical scavenging properties of low molecular weight. Evaluation of free radical scavenging activity of an. In vitro free radical scavenging and antidiabetic activity of. It is evident from the data that periploca aphylla and ricinus communis possess hydrogen donating capabilities and. Superoxide anion radical scavenging activity of cassia. Superoxide anion radical scavenging assay the method of ganga et al. The ic50 value table 1 of the test sample was centage inhibition of deoxyribose degradation. Dec 19, 2019 similarly, the superoxide free radical assay revealed the scavenging activity of eeap, eemd, and aemd which is comparable with standard ascorbic acid whereas aeap displayed significantly less scavenging activity over other tested extracts and standard fig. Free radical scavenging activity of the methanol extract was tested in three in vitro models, viz.
The superoxide radical scavenging activity was measured by the method of nishikimi et al. The presence of phenolic compounds prompted us to evaluate its antioxidant activity. Takeki hamasaki, taichi kashiwagi, toshifumi imada, noboru nakamichi, shinsuke aramaki, kazuko toh shinkatsu morisawa, hisashi shimak oshi, yoshio hisaeda, and sanetaka shirahata. This article is from iranian journal of pharmaceutical research. In vitro nitric oxide scavenging activity of methanol. Once formed, free radicals can start a chain reaction leading to formation of more free radicals. They showed a varying degree of dpph radical scavenging activity with ic50 values between 31. In addition, aafb melanin exhibited stronger scavenging activities on 2,2 diphenyllpicrylhydrazyl dpph radical, superoxide radical and hydroxyl radical with. These results suggest that tpl had the highest radical. Activity was calibrated using a standard material, lascorbic acid 23,4dihydro2,5,7,8. Invitro antioxidant and free radical scavenging activity of. The antioxidant and free radical scavenging activity of the ethanol extracts of melia dubia hiern. Flavonoid contents and free radical scavenging activity of.
Antioxidant and free radical scavenging activity of spondias. Dpph radical scavenging capacity of phenolic extracts from. Free radical scavenging activity of crude extracts and 4. A blank solution containing phosphate buffer, without h2o2 was prepared. Moreover, it had scavenging activity on hydroxyl radical compared with ascorbic acid fig. Table 1 shows the percentage of dpph radical scavenged by standards, that is, npropyl gallate and 3tbutyl4hydroxyanisole and different extracts of 2 plants at various concentrations. Antioxidant methods used were hydrogen peroxide scavenging h 2 o 2 assay and superoxide radical scavenging activity sod. Similarly, the superoxide free radical assay revealed the scavenging activity of eeap, eemd, and aemd which is comparable with standard ascorbic acid whereas aeap displayed significantly less scavenging activity over other tested extracts and standard fig. Superoxide anion radical scavenging activities of herbs and. The plant extracts exhibited strong antioxidant and radical scavenging activity on abts radical cation, dpph free radical, hydrogen peroxide. Eggplant epidermis extract exhibited the most potent hydroxyl radical scavenging and sodlike activities. What is the mechanism of superoxide anion free radical.
In this study we determined the free radical scavenging activity, total phenolic content, total oxidant level, and total antioxidant level in methanol and acetone extracts of t. Comparative study of abts radical scavenging activity and. Antioxidant and free radical scavenging activity of. The ec 50 values for the scavenging rates of dpph, hydroxyl and superoxide anion radicals were 3. Total phenol and total flavonoid content in extracts, which reason antioxidant and free radical scavenging activity, was. Several methods have been developed to assess the radical scavenging activity. Relationships between free radical scavenging and antioxidant. In vitro free radical scavenging and antidiabetic activity. The antioxidant activity of the aerial part extract of m. Dpph free radical scavenging activity of the extracts of the. Herbs and pastures, superoxide anion radical, scavenging activity, esr, hplc, altitudinal locations. The total phenolic content of the extracts was found to be higher in a. Hydroxyl radical scavenging activity hrsa of the extractives was determined by the method of klein et al. Preparation and evaluation of antioxidant activities of.
Ataturk university, faculty of sciences, department of chemistry, 25240erzurum, turkey. Antioxidants free fulltext antioxidant properties of. Acylhydrazide schiff bases 127 were evaluated for their in vitro dpph radical and superoxide anion scavenging activity. In the present study, methanolic fruit extract of c. Serotonin, antioxidant activity, metal chelating, reducing power, radical scavenging. The reactive oxygen ion superoxide is particularly important as the product of the oneelectron reduction of dioxygen o 2, which occurs widely in nature. The highest antioxidant and free radical scavenging ability of the fruit extract was observed at a concentration of 2500. The compound sodium nitroprusside is known to decompose in aqueous solution at physiological ph 7. Radical scavenging and antioxidant capacity of serotonin. Free radical scavenging activities of watersoluble extracts from some natural sources.208 1550 527 559 969 902 265 1387 1062 1116 720 1247 702 695 1342 1176 1104 1130 849 511 1124 360 892 1276 1362 1550 1533 588 1552 421 742 1343 1449 1004 1096 724 814 701 1260 1418 740 712 333 465 148 935 605 1013